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  • SquirtingTruth: Chemical Analysis of Female Ejaculate

    The most important piece of information when it comes to reassuring women that they are not urinating has been provided by chemical analysis of the fluid.

    1.  Numerous analysis have confirmed the presence of PSA (prostate-specific antigen) either in the fluids themselves or in the urine of women who did not ejaculate after orgasm.  This is important because:

    a) PSA is produced by the prostate gland and until this discovery, thought to be a male chemical produced by a male gland. 

    b) The male prostate gland is responsible for producing the male ejaculate (minus the sperm themselves).  The female prostate appears to have an identical role.

    c) It opened discussion of the connection between females who don't ejaculate and "retrograde ejaculation", where the ejaculate travels back up and into the bladder rather than out through the urethra.

    Studies which confirmed the presence of PSA include Whipple and Perry [1], and Dr. Cabello Santamaria [2].

    The study by F Cabello Santamaria analyzed urine for prostate-specific antigen (PSA) using Microparticle Enzyme Immunoassy and found that 75 per cent of the sample showed a concentration of PSA in post-orgasmic urine samples which was not present in pre-orgasmic urine samples. The fluid collected at the point of orgasm (distinct from the urine samples) showed the presence of PSA in 100 per cent of samples.


    2.  In addition to the PSA results, analysis carried out by Whipple and Perry in the early 80s established substantially higher levels of glucose.

    Edwin G. Belzer, Jr., Beverly Whipple and William Moger, co-researchers with Addiego, et al (1981), released a study in 1984 involving seven women mentioned in Addiego but whose results were not reported because the specimens were “collected in the absence of the research team.”  Because of concerns that the findings of Goldberg might discourage scientists from making the commitment of resources necessary to do further research, they decided to release the analyses of the specimens of the seven women.  Some of the tests were incomplete as there were insufficient quantities of ejaculate to compare with the urine specimen.  However, in three of the cases, acid phosphatase and glucose were reported to be present in greater quantities and urea and creatinine in lesser quantities in the ejaculate than in the urine sample.  In the other four cases, acid phosphatase was detected in the ejaculate but not in the urine.  These results were certainly more encouraging than those of Goldberg for those supporting the concept of “female ejaculation”.

    3.  In 1988, Milan Zaviacic, M. D., Ph.D., head of the Institute of Pathology, Comenius University Bratislava published a study of five women who were patients at a fertility department of a hospital of gynecology and obstetrics.  Total samples from one of the participants and one of four samples from a second participant were collected in the laboratory. The rest were collected at the homes of the women and transported to the laboratory in ice.  In four of the five cases, the samples were analyzed within three hours of collection, with the fifth subject’s specimens analyzed three months after collection.  The results in all five cases showed a higher concentration of fructose in the ejaculate sample than in the urine sample.  Zaviacic concluded that, “The determination of a higher fructose level in the ejaculate than in the urine suggests that the female ejaculate cannot be simply regarded as urine although urine is a regular component of the fluid of urethral expulsions at ejaculation.”   Further, he states, “The release of fructose into the fluid of expulsions emphasizes the need to broaden our notion on the mechanism of the urethral ejaculation phenomenon in the female.” [5]

    4. Chemical analysis also revealed substantially lower levels of creatinine and urea than in samples of urine from the same women.

    Cratinine and urea are two chemicals which define urine.  In these studies samples were taken from women before, during and after ejaculation.  The results showed that these two chemicals were quite high in the urine samples before and after ejaculation but that the level of these two chemicals was quite low in the ejaculatory fluid.

    Studies which revealed these results include Whipple and Perry [3], and Dr. Gary Shubach [4].



    1. Ladas, Alice Khan; Whipple, Beverly; and Perry, John, The G Spot and Other Discoveries about Human Sexuality. Holt, Rinehart and Winston, 1982.

    2. Cabello, F. (1997). Female ejaculation: Myth and reality. In J.J. Baras-Vass & M.Perez-Conchillo (Eds) Sexuality and Human Rights: Proceedings of the XIII World Congress of Sexology (pp.325-333) Valencia, Spain: E.C.V.S.A.

    3.  Edwin G. Belzer, Jr., Beverly Whipple and William Moger, co-researchers with Addiego, et al (1981)

    4.  Gary Schubach, Ed.D 2001, Urethral Expulsions During Sensual Arousal and Bladder Catheterization in Seven Human Females

    5.  Gary Schubach, Ed.D 2001, Urethral Expulsions During Sensual Arousal and Bladder Catheterization in Seven Human Females



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